mcf 10a (ATCC)

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ATCC mcf 10a
Mcf 10a, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 8168 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/c+normal+mammary+gland+epithelial+cell+line+mcf+10a/MCF+10A/custom%40crl-10317%4041907432
Average 99 stars, based on 8168 article reviews

mcf 10a - by Bioz Stars, 2026-10

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Article Title: Therapeutic agent for breast cancer comprising BIG3-PHB2 interaction-inhibiting peptide derived from PHB2
Article Snippet: .. Materials and Methods Cell Lines and Culturing Conditions Human breast cancer cell line MCF-7 was purchased from JCRB Cell Bank (Osaka, Japan) and maintained in MEM (Thermo Fisher Scientific) supplemented with 10% FBS (Nichirei Biosciences Inc., Tokyo, Japan), 1% Antibiotic/Antimycotic solution (Thermo Fisher Scientific, Waltham, Mass., USA), 0.1 mM NEAA (Thermo Fisher Scientific), 1 mM sodium pyruvate (Thermo Fisher Scientific), and 10 μg/mL insulin (Sigma, St. Louis, Mo., USA) under 5% CO2 at 37° C. Normal mammary gland epithelial cell line MCF-10A was purchased from American Type Culture Collection (ATCC, Manassas, Va., USA), and maintained in MEBM (Lonza) supplemented with a Single Quots kit (BPE, hydrocortisone, hEGF, insulin, gentamycin/amphoterin-B) (Lonza, Walkersville, Md., USA) and 100 ng/mL cholera toxin under 5% CO2 at 37° C. Cell Growth Assay Growth assay on MCF-7 was carried out by seeding cells into 48-well plates (2×104 cells/200 μL). .. First, on the next day after seeding, the medium was changed to phenol red-free DMEM/F12 (Thermo Fisher Scientific) supplemented with 10% FBS, 1% Antibiotic/Antimycotic solution, 0.1 mM NEAA, 1 mM sodium pyruvate, and 10 μg/mL insulin.

Article Title: Therapeutic agent for breast cancer comprising BIG3-PHB2 interaction-inhibiting peptide derived from PHB2
Article Snippet: .. Materials and Methods Cell lines and Culturing Conditions Human breast cancer cell line MCF-7 was purchased from JCRB Cell Bank (Osaka, Japan) and maintained in MEM (Thermo Fisher Scientific) supplemented with 10% FBS (Nichirei Biosciences Inc., Tokyo, Japan), a 1% Antibiotic/Antimycotic solution (Thermo Fisher Scientific, Waltham, MA, USA), 0.1 mM NEAA (Thermo Fisher Scientific), 1 mM sodium pyruvate (Thermo Fisher Scientific), and 10 μg/mL insulin (Sigma, St. Louis, MO, USA) under 5% CO2 at 37° C. Normal mammary gland epithelial cell line MCF-10A was purchased from American Type Culture Collection (ATCC, Manassas, Va., USA), and maintained in MEBM (Lonza) supplemented with a Single Quots kit (BPE, hydrocortisone, hEGF, insulin, gentamycin/amphoterin-B) (Lonza, Walkersville, MD, USA) and 100 ng/mL cholera toxin under 5% CO2 at 37° C. Cell Growth Assay Growth assay on MCF-7 was carried out by seeding cells into 48-well plates (2×104 cells/200 μL). ..

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